ELISA kit for quantification of human methionine sulfoxide reductase B in biological samples.
The oxidation of methionine at the sulfur atom leads to alternative epimers of methionine sulfoxide (MetO R form and S form). Methionine sulfoxide reductase B (MsrB), also known as SelX, is a selenoprotein that can reduce the R form of both free and protein-incorporated MetO to methionine. It has a crucial role in protecting cells against oxidative damages. MsrA reduces only the S epimer of MetO and MsrB reduces the R epimer of MetO in proteins. Although the catalytic mechanisms of MsrA and MsrB are similar, the two Msrs have no sequence identity and no structural similarity.
The NWLSS™ Methionine Sulfoxide Reductase B ELISA is a sandwich format Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human MsrB. Samples are pipetted into these wells. Nonbound MsrB and other components of the sample are removed by washing, then monoclonal antibody specific to MsrB added. In order to quantitatively determine the amount of MsrB present in the sample, Streptavidin Horseradish Peroxidase (HRP) is added to each microplate well. After another wash step, a TMB-substrate solution added to each well. Finally, a sulfuric acid stop solution is added and the resulting yellow colored product is measured at 450nm. The amount od MsrB in the sample can be determined by direct comparison with the standard curve generated in the assay.
View Assay Protocol
96 Well Sandwich ELISA
determination of Methionine sulfoxide reductase A (MsrB) in human serum,
plasma, cell lysates and cell culture supernatants
Human serum, plasma, cell lysates and cell culture supernatants.
|Storage and Stability:||
All kit components of this kit are stable at 2 to 8 ℃. Any unused reconstituted standard should be discarded or frozen at -70 ℃. Standard can be frozen and thawed one time only without loss of immunoreactivity.
1 Foil Pouch 96 well microplate precoated with anti-hu MsrB|
1 vial Human MsrB Standard (lyophilized) (1 Vial)
1 Bottle Sample/Standard Dilution Buffer (25mL)
1 vial 100X Secondary Antibody (Lyophilized) (1 Vial) (rabbit anti-hu MsrB)
1 Bottle Reagent Dilution Buffer (25mL)
1 vial 100X Anti-rabbit IgG-HRP Conjugate (150 mL)
1 Bottle TMB Substrate Solution (15 mL)
1 Bottle Stop Solution (1 N Sulfuric Acid) (15 mL)
2 Bottle 20X Concentrated Wash Buffer (25 mL ea)