The NWLSS™ Antioxidant Capacity kit measuring a sample's ability to reduce Cu++ to Cu+ as a means of assessing antioxidant status. The kit is useful as a simple method to quantify generic antioxidant capacity in many types of aqueous samples including serum, plasma, cell culture media and food extracts.
Not suitable for use with samples containing EDTA or other metal chelators.
Introduction
Oxidative stress is defined as a condition that exists when there is a lack of balance between pro-oxidants and antioxidants. Oxidative stress appears to be involved in the pathogenesis of several diseases including artherosclerosis, chronic inflammatory disease and cancer. Since oxidative stress can be harmful to organisms, intra-cellular, extra-cellular, enzymatic and non-enzymatic systems exist to mediate the condition. Lipid soluble antioxidants (most importantly vitamin E) and water-soluble antioxidants (uric acid, vitamin C, bilirubin, thiols and gluthathione) are a few of the antioxidants involved in these processes. Given the large number of antioxidant pathways, and their importance in regulation of an organism's redox status, it is important to be able to quantitatively estimate the capacity of antioxidants to reduce or antioxidant power within biological specimens.
Test Principle
The NWLSS™ Antioxidant Reductive Capacity Assay is based on the combined action of sample antioxidants to reduce Cu++ to Cu+. Cu+ reacts with bathocuproine (BC) to form a color complex with maximal absorbance at 480-490 nm. Measurement at 490 nm before and after addition of BC generates a net absorbance in direct relation to a sample's reductive capacity. Absorbance values obtained for samples are compared with a standard curve generated using Trolox. Alternatively, researchers may select a different standard of their choosing for use in the assay.
View Assay Protocol
| Catalog Number: |
NWK-ARC02
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| Format: |
100 Test, Colorimetric
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| Intended Use |
Quantitative measurement of antioxidant status in serum, plasma, cell
culture, food extracts and other aqueous biological samples
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| Sample Requirements: |
Serum, Plasma, Tissues, and other Biological Samples
Not recommended for use with EDTA Plasma samples or other samples where metal chelators may be present.
|
| Specificity: |
Specific for measurement of Antioxidant Reductive Capacity biological samples.
|
| Sensitivity: |
30 mM Trolox Equivalents
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| Storage and Stability: |
12 Months from date of manufacture when stored as specified (2-8C)
|
| Kit Contents: |
1 X 60 mL Dilution Buffer containing bathocuprione disulfonic acid.
1 X 5 mL Cu++ solution)
1 X 5 mL Stop Solution containing EDTA.
1 Vial Trolox Standard
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How does the NWK-ARC02 assay compare to assays such as FRAP and ORAC?
The NWK-ARC02 assay is somewhat analogous to the FRAP (Ferric Reducing Antioxidant Power) in that both utilize a metal ion as the reductive measurement. In the case of our kit the metal ion is Cu instead of Fe. For obvious reasons we couldn't replace the Ferric with Cupric and create a new acronym similar to FRAP to describe our assay so we chose to call it an Antioxidant Reductive Capacity assay.
The ORAC assay is different in that it measures the ability of antioxidants to quench a biologically relevant radical species. Our NWK-TAC01 assay is somewhat analogous to the ORAC assay as both measure an antioxidant response to a specific radical species and both report data as TROLOX equivalents. We couldn't replace the Ferric with Cupric and create a new acronym similar to FRAP to describe our assay so we chose to call it an Antioxidant Reductive Capacity assay.
