Product NWR-NEM01, N-ethylmaleimide (NEM) is sold as a sulfhydryl masking reagent. It can be used to render thiol containing reduced glutathione (GSH) unreactive when testing for oxidized glutathione (GSSG). When used as directed, this product provides sufficient NEM to treat 100 samples in preparation for assay of GSSG using NWLSS™ NWK-GSSG01 Assay kit or similar product type.
Product is compatible with samples from all species.
Rapid oxidation of GSH in non-reducing environments (e.g., outside of cells) presents a very real obstacle to accurate measurement of GSSG. Proper masking of reduced GSH requires immediate (if possible) treatment of samples with NEM in approximate 10 fold molar excess compared to expected sample GSH. Failure to treat samples in a timely manner allows for artifact GSH oxidation to occur leading to overestimation of GSSG.
If using with NWLSS™ GSSG or NWLSS™ GSHt/GSSG Assay kits, sample specific instructions for use can be found in the associated product inserts. Note: Excess NEM must be removed by extraction prior to assaying for GSSG.
NEM Reagent plus Diluent
Recommended as an effective thiol masking reagent
Effective when used with whole blood, tissue homogenates, RBC and other cell lysates.
Sulfydryl (thiol) masking reagent
|Storage and Stability:||
12 months from date of manufacture if stored at 2-8C