Glutathione Peroxidase (GPx) Activity Assay

NWLSS | Northwest Life Science Specialties, LLC.
Glutathione Peroxidase (GPx) Activity Assay
NWK-GPX01 $475.00 each

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A kinetic, rate based assay kit for quantification of glutathione peroxidase (GPx) in a variety of samples including blood products, tissues and cultured cells. This assay will work with any species.


Glutathione Peroxidase (GPx) (EC enzymes belong to a family of selenoproteins whose function is to catalyze the reduction of various peroxides. Four types of GPx (GPx-1 to 4) have been identified in mammalian species. GPx-1 is the classic intracellular form while GPx-2 to 4 are gastrointestinal, plasma and phospholipid hydroperoxide metabolizing forms respectively.

Test Principle

This assay is an adaptation of the method of Paglia and Valentine. Glutathione peroxidase (GPX) catalyzes the reduction of an organic peroxide (ROOH), oxidizing reduced glutathione (GSH) to form disulfde glutathione (GSSG). The oxidized glutathione is then reduced by glutathione reductase (GR) and b-nicotinamide adenine dinucleotide phosphate (NADPH) forming NADP+ (resulting in decreased absorbance at 340 nm) and recycling the GSH. Because glutathione peroxidase (GPx) is limiting, the decrease in absorbance at 340 nm is directly proportional to the glutthione (GPx) concentration.

Vitamin B12 deficiency results in severe oxidative stress, leading to memory retention impairment in Caenorhabditis elegans
Tomohiro Bito, Taihei Misaki, Yukinori Yabuta, Takahiro Ishikawa, Tsuyoshi Kawano, Fumio Watanabe,
Redox Biology ,  Available online 3 November 2016.

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Effect of Zirconium Dioxide Nanoparticles on Glutathione Peroxidase Enzyme in PC12 and N2a Cell Lines
Elham Asadpour, Hamid Reza Sadeghnia, Ahmad Ghorbani, and Mohammad Taher Boroushaki
Iran J Pharm Res. ,  2014 Autumn; 13(4): 1141–1148.

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Effects of N-Butanol And Ethylacetate Fractions of Indigofera Pulchra on Serum Lipid Peroxidation and Anti-Oxidant Enzymes on Normoglycaemic and Alloxan-Induced Diabetic Wistar Rats
Tanko Y, Mohammed A, Mabrouk M.A, Fatihu M.Y and Musa KY
Annals of Biological Sciences ,  2014; 2 (2):58-65

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Effect of selenium and grape seed extract on indomethacin-induced gastric ulcers in rats
Amr M. Abbas, Hussein F. Sakr
Journal of Physiology and Biochemistry, September 2013, Volume 69, Issue 3, pp 527-537

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Comparison of Deferoxamine and Methylprednisolone: Protective Effect of Pharmacological Agents on Lipid Peroxidation in Spinal Cord Injury in Rats
Dinc Cem MD, Iplikcioglu Ahmet Celal, Atabey Cem, Eroglu Ahmet MD, Topuz Kivanc, Ipcioglu Osman, Demirel Dilaver
Spine,  December 15, 2013 - Volume 38 - Issue 26

View Abstract

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For Research Use Only

Catalog Number: NWK-GPX01
Format: 192 tests presented as 2 X 96 well microtiter plate assays. Assay can also be performed in cuvettes with measurement via spectrophotometer for a 100 test yield per package
Sample Requirements: Tissues and cell lysates
Specificity: Glutathione Peroxidase
Sensitivity: LLD = 5.0 mU/mL by plate reader LLD = 1.0 mU/mL by spectrophotometer
Intended Use: Quantitative measurement of GPx activity
Storage and Stability: 12 months from date of manufacture if stored at 2-8C
Kit Contents: NADPH Reagent (containing GSH)
NADPH Diluent
H2O2 Reagent
Assay Buffer
96 Well Microplates
Random Citation | NWK-GPX01

Protective Effect of Chlorella vulgaris against Lead-Induced Oxidative Stress in Rat Brains 
HyeJun Yun, Inhye Kim, Sung-Hee Kwon, Ju-Seop Kang and Ae-Son Om 
Journal of Health Science Vol. 57 (2011) , No. 3 245-254 

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