Introduction
Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. This kit is for the quantification of 8-Isoprostane (15-isoprostane F2t), also known as 8-epi-PGF2aor 8-iso-PGF2a in urine samples. Levels of 8-Isoprostane (15-isoprostane F2t) in urine are useful for the non-invasive assessment of oxidant stress in vivo. 8-Isoprostane (15-isoprostane F2t) has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs, and may play a causative role in atherogenesis. Elevated isoprostane levels are associated with hepatorenal syndrome, rheumatoid arthritis, atherosclerosis, and carcinogenesis. This kit can be used for the quantification of free 8-Isoprostane (15-isoprostane F2t) in urine serum samples without the need for prior purification or extraction.
Test Principle
This assay utilizes a competitive enzyme-linked immunoassay (ELISA) strategy. Prior to assaying, all samples must be treated with an Enhancing Reagent. After treatment with Enhancing Reagent, 8-Isoprostane (15-isoprostane F2t) in the samples or standards is allowed to compete with 8-Isoprostane (15-isoprostane F2t) conjugated to horseradish peroxidase (HRP) for binding to a polyclonal antibody specific for 8-Isoprostane (15-isoprostane F2t) coated on a microplate. Later TMB substrate addition results in a blue color development that is inversely proportional to the quantity of 8-Isoprostane (15-isoprostane F2t) in the original samples or standards. Addition of an acid stop solution causes a color change to yellow where absorbance is read at 450 nM.
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